Multiphoton Microscopy

Multiphoton microscopy has become a powerful method for the nondestructive evaluation of deep-tissue cells and extracellular matrix (ECM) structures. By interacting with highly non-centrosymmetric molecular assemblies, the non-linear process called second harmonic generation has also proven to be an important diagnostic tool for the visualization of ECM compartments in situ with submicron resolution without the need for tissue processing.

Raman Spectroscopy

Raman spectroscopy has become a powerful diagnostic tool in the life sciences. Raman spectra allow assessment of the overall molecular constitution of biological samples, based on specific signals from proteins, nucleic acids, lipids, and carbohydrates. Measurements are non-invasive and do not require sample processing, making Raman spectroscopy a reliable and robust method with numerous applications in biomedicine.

Femtosecond Laser Optoporation

Ultrashort pulses of light can be used to create tiny, localized perforation in the membrane of cells. These transient perforations allow the delivery of DNA into the cell. Along with our collaborators, we use this technique to manipulate single cells. Although very much in its infancy, this technique can be optimized and automized to manipulate cells on a large scale.