ImageStreamX Mark II
The ImageStreamx mk II combines speed, fluorescence sensitivity and phenotyping abilities of flow cytometry with detailed imagery and functional insight of fluorescence microscopy and allows a discrimination of cells objectively and statistically based on their appearance. Overcoming the limitations of both techniques, the Imaging Flow cytometer opens the door to a range of novel applications such as Translocation, Co-localization, Immune synapse studies, Shape change and Chemotaxis, Internalization, Stem cell differentiation and many more.
The Core Facility
The Image Stream Core Facility is equipped with the most advanced Imaging Flow cytometer on the market. It is augmented with five lasers: 488 nm, 405 nm, 561 nm, 642 nm and SSC (785 nm). Two CCD cameras allow detection in 12 image channels with 3 different magnifications (20x, 40x, 60x). The EDF function extends the depth of field which can improve spot counting applications such as FISH, autophagy and nuclear translocation.
Analysis with IDEAS
IDEAS Image analysis software provides a large selection of features and masks to describe and analyze images by size, location, morphology, texture, phenotype, etc. We can guide you through the life-cycle of your project, from experimental setup and result analysis to composing graphics and figures for publication. Our experienced professional staff offers training and assistance for both imaging and analysis. Well-trained users may perform imaging and analysis without assistance.