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Imaging Flow Cytometry

What is Imaging Flow Cytometry?

ImageStreamX Mark II

The ImageStreamx mk II combines speed, fluorescence sensitivity and phenotyping abilities of flow cytometry with detailed imagery and functional insight of fluorescence microscopy and allows a discrimination of cells objectively and statistically based on their appearance. Overcoming the limitations of both techniques, the Imaging Flow cytometer opens the door to a range of novel applications such as Translocation, Co-localization, Immune synapse studies, Shape change and Chemotaxis, Internalization, Stem cell differentiation and many more.

The Core Facility

The Image Stream Core Facility is equipped with the most advanced Imaging Flow cytometer on the market. It is augmented with five lasers: 488 nm, 405 nm, 561 nm, 642 nm and SSC (785 nm). Two CCD cameras allow detection in 12 image channels with 3 different magnifications (20x, 40x, 60x). The EDF function extends the depth of field which can improve spot counting applications such as FISH, autophagy and nuclear translocation.

Analysis with IDEAS

IDEAS Image analysis software provides a large selection of features and masks to describe and analyze images by size, location, morphology, texture, phenotype, etc. We can guide you through the life-cycle of your project, from experimental setup and result analysis to composing graphics and figures for publication. Our experienced professional staff offers training and assistance for both imaging and analysis. Well-trained users may perform imaging and analysis without assistance.

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Imaging Flow Cytometry in a Short Video

Our Services

Recently performed ImageStreamX services

Cell-cell Interaction

Identification and Quantification of organized immunological synapses using Myeloid-derived suppressor cells co-cultured with splenocytes.

Cell-signaling

Transgenic GFP HEK 293 cells transfected with Cy3-labelled plasmids coding a guideRNA targeting GFP and an inactive RNA-guided nuclease.

Cell cycle and Mitosis

THP-1 cells stained with DRAQ5 to identify the nucleus.

Internalization – Phagocytosis

Internalization of S.aureus by PMNs.

Morphology and Shape change

Quantification of cell activation in monocytes. Shape change indicates the cell has responded to treatment.

Stem cell differentiation

The study of the maturation stages of embryonic stem cell derived cardiomyocytes (ESC-CMs).

Current scheduling

Calendar schedules for imaging and analysis

Publications

KSL Lab and Collaborative work

Stem Cell Reports paper published by Brauchle et al

One major obstacle to the application of stem cell-derived cardiomyocytes (CMs) for disease modeling and clinical therapies is the inability to identify the developmental stage ...

Work with the group from Stella Autenrieth

The challenging human pathogen Staphylococcus aureus has highly efficient immune evasion strategies for causing a wide range of diseases ...

Work with the group from Sandra Beer-Hammer

Fluorescently labeled Ly6G antibodies enable the tracking of neutrophils in mice, whereas purified anti-Ly6G rapidly depletes neutrophils from the circulation ...

Meet the Team

The ImageStreamX Core Facility Team
Prof. Katja Schenke-Layland
Principle Investigator
Simone Poeschel
Lead Technician
Simone Libscher
Lab Manager

Contact Us

We can be reached at normal office hours
Office Address
Department of Women‘s Health
Eberhard Karls University Tübingen
Silcherstrasse 7/1
Tübingen, 72076
Germany
Contact Information
Core Facility: +49 7071-29-85204
Administration: +49 7071-29-83068
simone.poeschel@med.uni-tuebingen.de